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Ellenberger and co-staff (eight) experienced intuited specifically such a movement of the OB domain after they solved the crystal construction in the much larger human DNA ligase I (HuLig1; 919 amino acids) bound to an adenylylated nick. Their landmark composition was the main to document the circumferential envelopment in the DNA duplex by a ligase clamp as well as resulting bending and distortion with the duplex that pressure The bottom pairs over the three′-OH side from the nick into an RNA-like A helical conformation (8). Very similar distortions ended up noticed from the ChVLig·DNA advanced (7).
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, shown are superimposed buildings of NAD+ plus the pyridopyrimidine inhibitor within their respective LigA complexes.
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